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Structure and Function of Biopolymers
High molecular weight complex of tyrosyl-tRNA synthetase from bovine liver
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The tyrosyl-tRNA synthetase in postribosomal bovine liver supernatant is included as a compound of the high molecular weight complex with Mr > 106 Da. Dissociation of tyrosyl-tRNA synthetase from this complex due to proteolysis proceeds through the intermediate labile complex with Mr≈240 kDa. The thermostability of tyrosyl-tRNA synthetase at this intermediate labile complex is much higher in comparison with free enzyme. The labile intermediate complex of bovine liver tyrosyl-tRNA synthetase possesses the nonspecific affinity for high molecular weight ribosomal RNA.
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