Biopolym. Cell. 1990; 6(6):96-99.
Gene-Engineering Biotechnology
The chromosome mutations in established chinese hamster fibroblasts, transfected by pBR322 recombinant plasmid carrying human insulin gene
1Rubashevsky E. L., 1Lukash L. L., 1Lysenko E. F., 1Neborachko L. N., 1Kirilenko S. I., 1Patskovsky Yu. V., 1Buzhievskaya T. I.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The pBR322 and pBR322ins (a recombinant plasmid carrying human insulin gene) plasmids have been shown to induce the chromosome aberrations both in early periods posttransfection of Chinese hamster established cells and in subsequent cell generations. So it is necessary to test the mutagenic properties of the DNA molecules produced both for genotherapy of human inherited diseases and for solution of other problems.


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