Biopolym. Cell. 1990; 6(6):93-96.
Gene-Engineering Biotechnology
Nitrogen-fixing Klebsiella species produce indole-3-acetic acid
1Kozyrovskaya N. A., 1Makitruk V. L., 2Ruckdashell E.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Lehrstuhl fur Genetik, Universitat Bayreuth


Nitrogen-fixing bacteria isolated from plant K. planticola and K. oxytoca were examined for their ability to produce indole compounds including the plant hormone indole-3-acetic acid. Indoles presented in culture filtrates were identified by thin-layer chromatography and quantitated by high-performance liquid chromatography. Bacteria produced IAA when liquid media were supplemented with L-tryptophan. Additional jndoles were found in culture filtrates: indole-3-lactic acid, indole-3-pyruvic acid, indole-3-acetaldehyde.


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