Biopolym. Cell. 1990; 6(6):89-92, 99.
Structure and Function of Biopolymers
Acidic polypeptides as inhibitors of the repair of double-strand DNA breaks
1Medvedev A. I., 1Revina G. I.
  1. Institute of Biological Physics, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

The ability of polypeptides consisting of aspartic and glutamic acids to inhibit the repair of double-strand DNA breaks induced in Y-irradiated Chinese hamster and HeLa cells was shown. A complete inhibiton of the double-strand DNA breaks repair was observed under the concentrations of 20 jimoles/1 (polyglutamic acid with molecular weight 2000–15000 daltons) and 100 umoles/1 (aspartylglutamic acid with moSccuar weight 1500-4500 daltons). Both polypeptides were low toxic at the given concentrations, their inhibitory effect being reversible.

References

[1] Cooper WJ, Cole A. Characterization studies of the rejoining of ?-particle and ?-ray induced double-strand acissions in mammalian DNA. Radiat Res. 1974; 59(1):262.
[2] Bryant PE, Blöcher D. The effects of 9-beta-D-arabinofuranosyladenine on the repair of DNA strand breaks in X-irradiated Ehrlich ascites tumour cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1982;42(4):385-94.
[3] A. c. 1418339, C12 N 15. 00. Inhibitor of DNA double strand breaks repair. AI. Medvedev. Bull. Izobreteniy. 1988; (31): 121.
[4] Medvedev AI, Mantsygin IuA, Revina GI, Veselova VA, Kublik LN. Effect of natural and synthetic acid polypeptides on normal and gamma-irradiated cells of the Chinese hamster. Radiobiologiia. 1978;18(6):821-8. Russian.
[5] Medvedev AI, Revina GI, Kuzin AM. Strongly acid polypeptides as inhibitors of the repair of single-stranded DNA breaks induced by gamma irradiation. Dokl Akad Nauk SSSR. 1983;270(1):232-5.
[6] Bradley MO, Kohn KW. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Res. 1979;7(3):793-804.
[7] Flerov GN, Barashenkov VS. Practical applications of heavy ion beams. Uspekhi fizicheskikh nauk. 1974; 114(2):351-73.
[8] Adams RLP. Cell Culture for Biochemists. Elsevier/North-Holland Biomedical Press. 1980; 292 p.