Biopolym. Cell. 1990; 6(6):83-89.
Structure and Function of Biopolymers
Isolation of specific mRNA using antiserum against the p35 protein of vaccinia virus envelope and mapping of the p35 protein gene
1Muravlev A. I., 1Chikaev N. A., 1Netesova N. A., 1Cheshenko N. V., 1Beklemishev A. B., 1Mertvetzov N. P.
  1. All-Union Research Institute of Molecular Biology
    Koltsovo, Novosibirsk Region, USSR

Abstract

An immunochemical isolation of a specific mRNA from vaccinia virus infected cells has been carried out using antiserum against the p35 protein of the virion envelope. The cDNA synthesized on this mRNA has been shown to hybridize specifically to the gene started at the distance of ~1150 base-pairs of the left end of the vaccinia virus DNA Hindlll A fragment. This gene encode a 26.200—Mr polypeptide. Treatment of the p2S protein with hitinase results in a decrease of its molecular weight to 28.5 kD. The gene mapped is supposed to encode the precursor of the vaccinia virus envelope glycoprotein p35.

References

[1] Mackett M, Smith GL. Vaccinia virus expression vectors. J Gen Virol. 1986;67 ( Pt 10):2067-82.
[2] Wittek R, H?nggi M, Hiller G. Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome. J Virol. 1984;49(2):371-8.
[3] Wittek R, Richner B, Hiller G. Mapping of the genes coding for the two major vaccinia virus core polypeptides. Nucleic Acids Res. 1984;12(12):4835-48.
[4] Rosel J, Moss B. Transcriptional and translational mapping and nucleotide sequence analysis of a vaccinia virus gene encoding the precursor of the major core polypeptide 4b. J Virol. 1985;56(3):830-8.
[5] Hirt P, Hiller G, Wittek R. Localization and fine structure of a vaccinia virus gene encoding an envelope antigen. J Virol. 1986;58(3):757-64.
[6] Weir JP, Moss B. Use of a bacterial expression vector to identify the gene encoding a major core protein of vaccinia virus. J Virol. 1985;56(2):534-40.
[7] Niles EG, Seto J. Vaccinia virus gene D8 encodes a virion transmembrane protein. J Virol. 1988;62(10):3772-8.
[8] Maa JS, Esteban M. Structural and functional studies of a 39,000-Mr immunodominant protein of vaccinia virus. J Virol. 1987;61(12):3910-9.
[9] Rodriguez JF, Esteban M. Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodalton fusion protein. J Virol. 1987;61(11):3550-4.
[10] Rodriguez JF, Kahn JS, Esteban M. Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene. Proc Natl Acad Sci U S A. 1986;83(24):9566-70.
[11] Muravlev AI, Poryvaeva VA, Reshetnikov SS. Changes in antigenic properties of the p35 protein of vaccinia virus in various protein fractions of the virion with the use of monospecific antisera. Mol Gen Mikrobiol Virusol. 1989;(9):3-7.
[12] Muravlev AI, Netesova NA, Tikhonov VIa, Malygin EG. Analysis of structural proteins and products of cell-free translation of vaccinia virus of mRNA using mono-specific antibodies. Mol Gen Mikrobiol Virusol. 1989;(10):19-24.
[13] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.
[14] Cabrera CV, Esteban M. Procedure for purification of intact DNA from vaccinia virus. J Virol. 1978;25(1):442-5.
[15] Netesova NA, Muravlev AI, Chikaev NA. Immunochemical analysis of the products of translation of mRNA hybridized with the left HindIII-A-Sa1-fragment of vaccinia virus DNA. Mol Gen Mikrobiol Virusol. 1989;(11):21-3.
[16] Weinrich SL, Hruby DE. A tandemly-oriented late gene cluster within the vaccinia virus genome. Nucleic Acids Res. 1986;14(7):3003-16.
[17] Bajsz?r G, Wittek R, Weir JP, Moss B. Vaccinia virus thymidine kinase and neighboring genes: mRNAs and polypeptides of wild-type virus and putative nonsense mutants. J Virol. 1983;45(1):62-72.
[18] Boisvert J, Yamamoto T. Purification and location of the major glycoprotein of vaccinia virus. Microbios. 1976;16(63):55-72.
[19] Lee CY, Scocca JR. A common structural unit in asparagine-oligosaccharides of several glycoproteins from different sources. J Biol Chem. 1972;247(18):5753-8.
[20] Gordon J, Kovala T, Dales S. Molecular characterization of a prominent antigen of the vaccinia virus envelope. Virology. 1988;167(2):361-9.
[21] Rosel JL, Earl PL, Weir JP, Moss B. Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment. J Virol. 1986;60(2):436-49.