Biopolym. Cell. 1990; 6(4):92-96.
Gene-Engineering Biotechnology
Expression in Escherichia coli of the human angiogenin gene translationally fused with the N-terminal fragment of β-galactosidase
1, 2Kovalenko S. P., 1Mertvetsov N. P.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. Institute of Therapy, Siberian Branch of Medical Sciences of the USSR
    Novosibirsk, USSR
The phage M13mp8 earring a DNA fragment coding for fusion protein angiogenin-α-peptide of β-galactosidase was obtained by oligonucleotide directed mutagenesis technique from the M13mp8 phage with a cloned synthetic human angiogenin gene. The synthesis of peptide with, α-donor activity in the phage Ml-1-infected cells was demonstrated. The amount of the fusion protein was estimated at the different stages of E. coll cell growth after the phage infection.