Biopolym. Cell. 1990; 6(3):99-102.
Short Communications
Preparation and purification of E. coli Tem-1β-lactamase
1Sorochinskaya T. V., 1Chernykh S. I., 1Levitina T. L., 1Rodnin N. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The phage-technology for supersynthesis of β-lactamase TEM-1 in E. coli cells has been worked out. It has permited obtaining (1–2) ·106 units of enzyme activity per ml of cultural fluid. The method for wide-scale β-lactamase preparation is suggested, that is much simplier as against those known before. The enzyme recovery is about 45 %. Our preparation is electrophoretically homogenous and may be used in immuno-enzyme assay without further purification.

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