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Preparation and purification of E. coli Tem-1β-lactamase
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
The phage-technology for supersynthesis of β-lactamase TEM-1 in E. coli cells has been worked out. It has permited obtaining (1–2) ·106 units of enzyme activity per ml of cultural fluid. The method for wide-scale β-lactamase preparation is suggested, that is much simplier as against those known before. The enzyme recovery is about 45 %. Our preparation is electrophoretically homogenous and may be used in immuno-enzyme assay without further purification.
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