Biopolym. Cell. 1990; 6(3):84-89.
Gene-Engineering Biotechnology
Malacosoma neustria nuclear polyhedrosis virus expression vector
1Strokovskaya L. I., 1Kikhno I. M., 1Veselovsky O. V., 1Skuratovskaya I. N., 1Miryuta N. Yu., 1Petrenko A. I., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The β-galactosidase gene of E. coli was inserted in phase with coding sequence of the Malacoscma neustria nuclear polyhedrosis virus (MnNPV) polyhedrin gene. The fusion gene was inserted into the MnNPV genome by cotransfection of recombinant plasmid pMPEA-3 gal and wild-type MnNPV DNA. Infection of Antheraea pernyi cells (MCAp-1) by the resulting recombinant MnNPV gal 3 has shown high level of expression of polyhedrin-β-galactosidase fusion protein, no synthesis of polyhedrin being revealed.


[1] Smith GE, Summers MD, Fraser MJ. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol. 1983;3(12):2156-65.
[2] Luckow VA, Summers MD. Trends in the Development of Baculovirus Expression Vectors. Bio/Technology.1988;6(1):47–55.
[3] Skuratovskaya IN, Strokovskaya LI, Komissarenko SV, Mendeleva NV. Structure and functioning of the nuclear polyhedrosis virus genome in Melacosoma neustria. Tsitol Genet. 1986; 20(1):31-6.
[4] Strokovskaya LI, Veselovsky OV, Kikhno IM, Mirjuta NYu. Cloning of the Malakosoma neustria nuclear polyhedrosis virus polyhedrin gene. Biopolym. Cell. 1990; 6(3):81-4.
[5] Sukhorada EM, Kanyuka VYu, Miloserdova VD. Reproduction of silkworm ringed nuclear polyhedrosis virus by prolonged passaging in insect transplanted cells lines. Entomopathogenic viruses and their practical significance. Kyiv, Naukova dumka, 1981; 44-50.
[6] Pennock GD, Shoemaker C, Miller LK. Strong and regulated expression of Escherichia coli beta-galactosidase in insect cells with a baculovirus vector. Mol Cell Biol. 1984;4(3):399-406.
[7] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.
[8] Cherepenko EI, Martynenko EP. A simple method for baculovirus DNA isolation. Mol Biol (Mosk). 1985; 19(6):1519-24.
[9] Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977;74(2):560-4.
[10] Chuvpilo SA, Kravchenko VV. A solid phase method of determining DNA sequence. Bioorg Khim. 1983;9(12):1634-7.
[11] Summers MD., Smith GA. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas: Col. Stat., 1987. 56 p.
[12] Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.
[13] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[14] Rohrmann GF. Polyhedrin structure. J Gen Virol. 1986;67 ( Pt 8):1499-513.