Biopolym. Cell. 1990; 6(3):81-84.
Gene-Engineering Biotechnology
Cloning of the Malakosoma neustria nuclear polyhedrosis virus polyhedrin gene
1Strokovskaya L. I., 1Veselovsky O. V., 1Kikhno I. M., 1Mirjuta N. Yu.
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
This investigation is the first step of baculovirus expressive vector construction using the Malakosoma neustria nuclear polyhedrosis virus (MnNPV). The polyhedrin gene has been localized in the MnNPV genome. Recombinant plasmid has been constructed carryinga viral DNA fragment containing a polyhedrin gene with flanking DNA nucleotide sequences. This plasmid can be used for the transfer vector construction.
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