Biopolym. Cell. 1990; 6(3):77-80.
Gene-Engineering Biotechnology
Genetic transformation of pea plants mediated by Agrobacterium tumefaciens
1Zubko E. I., 1Kuchuk N. V., 2Tumanova L. G., 2Vikonskaya N. A., 1Gleba Yu. Yu.
  1. N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Ecological Genetics, Academy of Sciences of the Moldavian SSR
    Kishinev, USSR

Abstract

The actively regenerating lines of pea (Pisum sativum L.) on the hormone-free B5 medium are selected after cocultivation of shoot and leave segments with the «shooty» mutant of Agrobacterium tumcfaciens pGV 2206. These lines retain the regeneration ability at least for 18 months. Normalized but phenotypically different shoots are obtained. DNA-DNA hybridization analysis has revealed the presence of the structural NPT II gene in the BamH1-digested total DNA isolated from pea regenerants. Transgenic shoots grafted on the normal pea plants gave both flowers and seeds.

References

[1] St Schell J. Transgenic plants as tools to study the molecular organization of plant genes. Science. 1987;237(4819):1176-83.
[2] Piruzyan ES. Basics of plant genetic engineering. Moscow, Nauka, 1988; 340 p.
[3] Willmitzer L. The use of transgenic plants to study plant gene expression. Trends Genet. 1988;4(1):13-8. Review.
[4] Botterman J, Leemans J. Engineering herbicide resistance in plants. Trends Genet. 1988;4(8):219-22. Review.
[5] Leemans J, Shaw C, Deblaere R, De Greve H, Hernalsteens JP, Maes M, Van Montagu M, Schell J. Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet. 1981;1(2):149-64.
[6] Greve HD, Leemans J, Hernalsteens J-P, Thia-Toong L, Beuckeleer MD, Willmitzer L, et al. Regeneration of normal and fertile plants that express octopine synthase, from tobacco crown galls after deletion of tumour-controlling functions. Nature. 1982;300(5894):752–5.
[7] Leemans J, Deblaere R, Willmitzer L, De Greve H, Hernalsteens JP, Van Montagu M, Schell J. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J. 1982;1(1):147-52.
[8] Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50(1):151-8.
[9] Deak M, Kiss GB, Koncz C, Dudits D. Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep. 1986;5(2):97-100.
[10] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.
[11] Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugawara Y. Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet. 1986;204(2):204–7.
[12] Lichtenstein C. P., Draper J. Genetic engineering of plants. DNA cloning: a practical appoach. Ed . D. M. Glover. Washington: IRL press, 1985. V. 2:78.
[13] Methodical recommendations on micro cloning pea plants in tissue culture in vitro. M., 1988. 42p.
[14] Barry GF, Rogers SG, Fraley RT, Brand L. Identification of a cloned cytokinin biosynthetic gene. Proc Natl Acad Sci U S A. 1984;81(15):4776-80.