Biopolym. Cell. 1990; 6(3):70-74.
Cell Biology
Role of the nucleocytoplasmic ratio in regulation of the mammalian development. Development of the embryos with the doubled cytoplasm volume
1Evsykov S. V., 1Morozova L. M., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Certain blastomeres of the two-cell mouse embryos and blastomeres with the doubled cytoplasm volume have been compared as to their development in vitro. A decrease of the nucleocytoplasmic ratio had no effect on the rate of embryo cleavage, but the blastocoele appearance was 10 h delayed with more cleavage divisions than in control. The presented hypothesis suggests that the change of cellular morphogenetic programs requires definite concentration of substances — products of the previously realized program.

References

[1] Evsykov SV, Morozova LM, Solomko AP. Role of the nucleocytoplasmic ratio in the regulation of the mammal development. Development of zygotes with a decreased cytoplasm volume Biopolym Cell. 1989; 5(5):87-93.
[2] Bolton VN, Oades PJ, Johnson MH. The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo. J Embryol Exp Morphol. 1984;79:139-63.
[3] Smith RK, Johnson MH. DNA replication and compaction in the cleaving embryo of the mouse. J Embryol Exp Morphol. 1985;89:133-48.
[4] Dean WL, Rossant J. Effect of delaying DNA replication on blastocyst formation in the mouse. Differentiation. 1984;26(2):134-7.
[5] Alexandre H. The utilization of an inhibitor of spermidine and spermine synthesis as a tool for the study of the determination of cavitation in the preimplantation mouse embryo. J Embryol Exp Morphol. 1979;53:145-62.
[6] Kaufman MH, Sachs L. Complete preimplantation development in culture of parthenogenetic mouse embryos. J Embryol Exp Morphol. 1976;35(1):179-90.
[7] Modli?ski JA. Preimplantation development of microsurgically obtained haploid and homozygous diploid mouse embryos and effects of pretreatment with Cytochalasin B on enucleated eggs. J Embryol Exp Morphol. 1980;60:153-61.
[8] Howlett SK, Barton SC, Surani MA. Nuclear cytoplasmic interactions following nuclear transplantation in mouse embryos. Development. 1987;101(4):915-23.
[9] Tarkowski AK, Rossant J. Haploid mouse blastocysts developed from bisected zygotes. Nature. 1976;259(5545):663-5.
[10] McGrath J, Solter D. Nucleocytoplasmic interactions in the mouse embryo. J Embryol Exp Morphol. 1986;97 Suppl:277-89.
[11] Witkowska A. Parthenogenetic development of mouse embryos in vivo. I. Preimplantation development. J Embryol Exp Morphol. 1973;30(3):519-45.
[12] Tarkowski A. K. Experimental studies on regulation in the development of isolated blastomeres of mouse eggs. Acta theriol. 1959; 3(11):191-267.
[13] Hoppe PC, Whitten WK. Does X chromosome inactivation occur during mitosis of first cleavage? Nature. 1972;239(5374):520.
[14] Tsunoda Y, McLaren A. Effect of various procedures on the viability of mouse embryos containing half the normal number of blastomeres. J Reprod Fertil. 1983;69(1):315-22.
[15] Hogan B, Constantini F, Lacy E. Manipulating the mouse embryo. A laboratory manual. New York : Cold Spring Harbor Lab., 1986; 350 p.
[16] McGrath J, Solter D. Nuclear transplantation in the mouse embryo by microsurgery and cell fusion. Science. 1983;220(4603):1300-2.
[17] Giles RE, Ruddle FH. Production of Sendai virus for cell fusion. In Vitro. 1973;9(2):103-7.
[18] Harris H, Watkins JF. Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species. Nature. 1965;205:640-6.
[19] O'brien MJ, Critser ES, First NL. Developmental potential of isolated blastomeres from early murine embryos. Theriogenology. 1984;22(5):601-7.
[20] Rands GF. Cell allocation in half- and quadruple-sized preimplantation mouse embryos. J Exp Zool. 1985;236(1):67-70.
[21] Maro B., Howlett S. K., Johnson M. H. Cellular and molecular interpretation of mouse early development: the first cell cycle. Gametogenesis and the early embryo. Ed. J. G. Gall-N e w York: A. R. Liss, 1986:389-407.
[22] Petzoldt U, Muggleton-Harris A. The effect of the nucleocytoplasmic ratio on protein synthesis and expression of a stage-specific antigen in early cleaving mouse embryos. Development. 1987;99(4):481-91.
[23] Johnson MH. The molecular and cellular basis of preimplantation mouse development. Biol Rev Camb Philos Soc. 1981;56(3):463-98.
[24] Borsuk E, Ma?ka R. Behavior of sperm nuclei in intact and bisected metaphase II mouse oocytes fertilized in the presence of colcemid. Gamete Res. 1988;20(3):365-76.
[25] Kidder GM, McLachlin JR. Timing of transcription and protein synthesis underlying morphogenesis in preimplantation mouse embryos. Dev Biol. 1985;112(2):265-75.
[26] Levy JB, Johnson MH, Goodall H, Maro B. The timing of compaction: control of a major developmental transition in mouse early embryogenesis. J Embryol Exp Morphol. 1986;95:213-37.
[27] Rott NN. New data on nuclear transplantation in mammals. Ontogenez. 1987;18(4):341-4.
[28] Solter D. Inertia of the embryonic genome in mammals. Trends Genet. 1987;3:23–7.
[29] Rott NN, Sheveleva GA. Changes in the rate of cell divisions in the course of early development of diploid and haploid loach embryos. J Embryol Exp Morphol. 1968;20(2):141-50.
[30] Newport J, Kirschner M. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage. Cell. 1982;30(3):675-86.
[31] Tarkowski A. K. Nucleo-cytoplasmic interactions in oogenesis and early embryogenesis in the mouse. Embryonic Development. Genetic Aspects. Eds M. M. Burger, R. Weber-New York: A. R. Liss, 1982:407-416.
[32] Stice SL, Robl JM. Nuclear reprogramming in nuclear transplant rabbit embryos. Biol Reprod. 1988;39(3):657-64.
[33] Surani MA, Barton SC, Norris ML. Nuclear transplantation in the mouse: heritable differences between parental genomes after activation of the embryonic genome. Cell. 1986;45(1):127-36.
[34] Prather RS, First NL. Chimerization of highly asynchronous murine blastomeres: developmental alteration? Gamete Res. 1988;19(4):359-67.
[35] Larionov OA, Platonov ES, Mironova OV, Vasil'eva IA, Koniukhov BV. Transplantation of the cell nuclei of the crystalline lens anlage into mouse zygotes and the expression of alpha-crystallin genes. Zh Obshch Biol. 1988;49(2):263-70.