Biopolym. Cell. 1990; 6(1):88-90,106.
Studies in expression of human exogenous insulin gene under control of regulatory region of hsp70 dr. melanogaster gene in human fibroblasts
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
A vehicle has been constructed where the human insulin gene is under control of the regulatory region of hspJO Dr. melanogaster gene. The expression of this gene in human fibroblasts under the effect of heat shock has been studied. It is shown that four-fold increase in the expression of the human insulin gene is observed in response to heat shock.
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References
[1]
Schlesinger M. J., Kelly P. M., Miperti G. Properties of three major heat shock proteins and their antibodies. Heat shock from bacteria to man. New York : Cold Spring Harbor Lab., 1982:243-246.
[2]
Goldschmidt-Clermont M. Two genes for the major heat-shock protein of Drosophila melanogaster arranged as an inverted repeat. Nucleic Acids Res. 1980;8(2):235-52.
[3]
Craig EA, McCarthy BJ, Wadsworth SC. Sequence organization of two recombinant plasmids containing genes for the major heat shock-induced protein of D. melanogaster. Cell. 1979;16(3):575-88.
[4]
Pelham HR. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982;30(2):517-28.
[5]
Dreano M, Brochot J, Myers A, Cheng-Meyer C, Rungger D, Voellmy R, Bromley P. High-level, heat-regulated synthesis of proteins in eukaryotic cells. Gene. 1986;49(1):1-8.
[6]
Asano M, Nagashima H, Iwakura Y, Kawade Y. Interferon production under the control of heterologous inducible enhancers and promoters. Microbiol Immunol. 1988;32(6):589-96.
[7]
Neborachko LN, Lukash LL, Troyanovsky BM, Varzanova IS, Buzhievskaya TI, Kordium VA. Studies in possibility of expression of exogenic human insulin gene in cultured mammalian fibroblasts. Biopolym Cell. 1989; 5(2):58-61.
[8]
An G, Hidaka K, Siminovitch L. Expression of bacterial beta-galactosidase in animal cells. Mol Cell Biol. 1982;2(12):1628-32.