Biopolym. Cell. 1989; 5(6):78-82.
Cell Biology
Agglutination of rat thymocytes induced by aggregated Concanavalin A
1Timoshenko A. V., 1Cherenkevich S. N.
- Lenin Byelorussian State University
Minsk, USSR
Abstract
The effect of NADH, peroxidase and catalase precipitated in the presence of concanavalin A (Con A) on the Con A-induced agglutination of rat thymocytes has been analyzed by the light transmission method. The aggregated Con A causes a considerable acceleration of agglutination compared with the action of native Con A. The rate of thymocyte agglutination depends on Con A-aggregated size. These results testify to the key role of the membrane clusters formation in the provision of Con A-induced cell1 agglutination.
Full text: (PDF, in Russian)
References
[1]
Frants Kh. Lectins: properties, functions, and possible applications. Zh Mikrobiol Epidemiol Immunobiol. 1980;(1):3-10.
[2]
Metakovskii EV. Mechanism of intercellular contacts and the change in cell membranes during differentiation. Mol Biol (Mosk). 1981;15(4):753-67.
[3]
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. Molecular biology of the cell. New York and London, Garland Publishing, Inc., 1983.
[4]
Zengbush P. Molecular and Cellular biology. Moscow, Mir, 1982; Vol. 3:320 p.
[5]
Mastromarino P, Neri G, Serra A, Walborg EF Jr. Role of cell surface receptor mobility in concanavalin A-induced agglutination of Novikoff hepatoma and normal rat liver cells. J Cell Sci. 1980;42:169-82.
[6]
Roth J. The lectins: molecular probes in cell biology and membrane research. Exp Phathol. 1978. Suppl. 3:1-686.
[7]
Timoshenko AV, Cherenkevich SN. Regulation by concanavalin A state redox systems of the thymocytes plasma membrane. Tsitologiya. 1987; 29(9):1107.
[8]
Capo C, Garrouste F, Benoliel AM, Bongrand P, Ryter A, Bell GI. Concanavalin-A-mediated thymocyte agglutination: a model for a quantitative study of cell adhesion. J Cell Sci. 1982;56:21-48.
[9]
Loor F. Lectin-induced lymphocyte agglutination. An active cellular process? Exp Cell Res. 1973;82(2):417-25.
[10]
Pfeifer RW, Patterson RM. Modulation of lectin-stimulated lymphocyte agglutination and mitogenesis by estrogen metabolites: effects on early events of lymphocyte activation. Arch Toxicol. 1986;58(3):157–64.
[11]
Timoshenko AV, Cherenkevich SN, Samal' AB. Role of reactive oxygen sPecies in a concanavalin A-induced lymPhocyte agglutination. Vestn. Belarusian university. Ser. 2. 1986; (3):47-50.
[12]
Rule GS, Kruuv J, Lepock JR. Membrane lipid fluidity as rate limiting in the concanavalin A-mediated agglutination of pyBHK cells. Biochim Biophys Acta. 1979;556(3):399-407.
[13]
Singer JA, Morrison M. Effect of adenosine on concanavalin A agglutination of human erythrocytes. Biochim Biophys Acta. 1980;598(1):40-50.
[14]
Ng MH, Ng WS. Concanavalin A receptor capping and blastogenesis in human lymphocytes: inhibition by egg lecithin. Cytobios. 1981;32(125):39-46.
