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Structure and Function of Biopolymers
Studies in correlation between poly(U) misreading and efficiency of poly(dT) translation in the cell-free protein-synthesizing systems of E. coli
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
The efficiency of poly(dT) translation and misreading of poly(U) has been studied in cell-free systems from wild-type E. coli and streptomycin-resistant mutants with altered ribosome protein S12. The data show that there is a positive correlation between poly(U) misreading and efficiency of poly(dT) translation. Conditions promoting misreading of poly(U): an increase in the concentration of neomycin, kanamycin, streptomycin antibiotics, of Mg2+ ions stimulate poly(dT) translation as well. A series of antibiotics activities in promotion of both poly(U) misreading and poly(dT) translation is the same: neo-, mycin> kanamycin > streptomycin. The more accurate ribosomes with altered protein S12 are less efficient in translation of poly(dT). T he data obtained are in good agreement with the hypothesis on stereospecific stabilization of codon-anticodon complexes on the ribosome.
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