Biopolym. Cell. 1989; 5(4):98-102.
Gene-Engineering Biotechnology
DNA synthesis in intact maize mitochondria treated by pBR bacterial vector plasmids
1Konstantinov Yu. M., 1Podsosonny V. A., 1Lutsenko G. N., 2Rivkin M. I.
  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Academy of Sciences of the USSR
    Irkutsk, USSR
  2. Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


Fractions of high-molecular weight («chromosomal») and plasmid-like DNA from the isolated maize seedling mitochondria are synthesized with different rate. DNA of bacterial vector pBR327 and pBR322 plasmids is shown to be translocated into inner space of isolated mitochondria. Radioautographic analysis has been used to find the matrix activity of the supercoiled pBR322 plasmid to synthesize DNA in the system of intact maize mitochondria. The model system described is assumed to be used to study th« problems of replication and transcription of recombinant plasmid DNA in the plant mitochondria.


[1] Grierson D, Covey SN. Plant molecular biology. Glasgow; London: Blackis, 1984. 176 P.
[2] Esser K, Kuck U, Stahl U, Tudzynski P. Cloning vectors of mitochondrial origin for eukaryotes: A new concept in genetic engineering. Curr Genet. 1983;7(4):239-43.
[3] Levings CS., III. Cytoplasmic male sterility. Genet , eng. of Plants: Agr. Perspect. Proc. symp. New York; London, 1983:81-92.
[4] Kemble RJ, Bedbrook JR. Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasm. Nature. 1980;284(5756):565–6.
[5] Bedinger P, Walbot V. DNA synthesis in purified maize mitochondria. Curr Genet. 1986;10(8):631-7.
[6] Carlson JE, Brown GL, Kemble RJ. In organello mitochondrial DNA and RNA synthesis in fertile and cytoplasmic male sterile Zea mays L. Curr Genet. 1986;11(2):151–60.
[7] Gleba YuYu, Sytnik KM. ProtoPlast fusion and genetic engineering of higher Plants. Kiev, Naukova Dumka, 1982; 102 P.
[8] A somatic cell genetics methods. Ed. J Shay. Moscow, Mir, 1985; Vol. 1. 312 P.
[9] Kazakova TB, Babich SG, Golovina GI, Mel'nikova MP, Tzimbalenko NV. Replication of plasmid chimera pBR-mtB-A containing rat liver mtDNA fragment in Escherichia coli polA cells. Plasmid. 1982;8(1):1-8.
[10] Voinikov VK, Luzova GB, Lemzyakov VP. Fatty acid content and activity of mitochondria in winter rye subjected to chilling. Fiziologiia rasteniy. 1981; 28(1):18-26.
[11] Budker VG, Godovikov AA, Naumova LP, Slepneva IA. Interaction of polynucleotides with natural and model membranes. Nucleic Acids Res. 1980;8(11):2499-515.
[12] Budker VG, Gorokhova OE, Sokolov AV. DNA translocation across model phospholipid membranes. Dokl Akad Nauk SSSR. 1984;278(2):479-82.
[13] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold SPring Harbor, 1982; 545 P.
[14] ter Schegget J, Borst P. DNA synthesis by isolated mitochondria. I. Effect of inhibitors and characterization of the product. Biochim Biophys Acta. 1971;246(2):239-48.
[15] Kemble RJ, Gunn RE, Flavell RB. Classification of Normal and Male-Sterile Cytoplasms in Maize. II. Electrophoretic Analysis of DNA Species in Mitochondria. Genetics. 1980;95(2):451-8.
[16] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.
[17] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[18] Kuzmin EV, Levchenko IV. S1 plasmid from cms-S-maize mitochondria encodes a viral type DNA-polymerase. Nucleic Acids Res. 1987;15(16):6758.