Biopolym. Cell. 1989; 5(2):94-97.
Short Communications
Studies in replication of plasmids containing a complete SV40 genome in permissive mammalian cells
1Landau S. M., 1Sasina L. K., 2Shlyankevich M. A., 2Dreeze O. B.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Research Institute of Carcinogenesis, All-Union Cancer Research Center, Academy of Medical Sciences of the USSR
    Moscow, USSR

Abstract

After simultaneous infection of permissive cells with SV40 and transfection with plasmids containing the SV40 genome and "poison sequences", replication of plasmids reaches the maximum level (106 copies per infected cell). When the plasmids are transfected 24 h after post-infection of cells with SV40, the replication level decreases several times and approximately corresponds to the level of their replication in COS cells.

References

[1] Lusky M, Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981;293(5827):79-81.
[2] Sasina LK, Landau SM, Kordium VA. Construction and analysis of two-replicon hybrid plasmids containing the genome of simian papovavirus SV40. Mol Gen Mikrobiol Virusol. 1986;(1):26-30.
[3] Suissa M. Spectrophotometric quantitation of silver grains eluted from autoradiograms. Anal Biochem. 1983;133(2):511-4.
[4] Landau SM, Sasina LK, Chashchin NA, Chashchina LI. Studies in replication of plasmids containing SV40 genome in mammalian cells. Biopolym Cell. 1987; 3(3):134-6.
[5] Tsui LC, Breitman ML, Siminovitch L, Buchwald M. Persistence of freely replicating SV40 recombinant molecules carrying a selectable marker in permissive simian cells. Cell. 1982;30(2):499-508.