Biopolym. Cell. 1989; 5(2):69-73, 106.
Structure and Function of Biopolymers
Changes in functional properties of restriction enzymes under the influence of (2'-5')Oligoadenylates in vitro
1Tkachuk Z. Yu., 1Tkachuk L. V., 2Kvasyuk E. I., 2Zaitseva G. V., 2Kalinichenko E. N., 2Mikhailopulo I. A., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Bioorganic Chemistry, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR

Abstract

It is found that «core» trimers of (2'-5') oligoadenylic acid, viz., (A2'p)2A and-(3'dA2'p)23'dA, exert an influence on functional properties of restriction enzymes EcoRl, BamHl, and Hindlll. The presence of (2'-5') trimers in the reaction mixture containing DNA and the restriction enzyme results in (i) an inhibition of the restriction reaction, (ii) site-specific restriction of DNA λ, or (iii) nonspecific hydrolysis of DNA λ. In the last case restriction enzymes function as nonspecific DNAses.

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