Biopolym. Cell. 1989; 5(1):58-66.
Gene-Engineering Biotechnology
Unidirectional orientation of Escherichia coli rplKAJL·rpoBC operon fragments on a multicopy pUC plasmid
1Paton E. B., 1Kroupskaya I. V., 1Zhyvoloup A. N.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


A series of recombinant pUC plasmids was constructed to determine the functional domains of the regulatory ribosomal L10 protein of Escherichia coli, encoded by the rplJ gene. The possibility and peculiarities of cloning of the E. coli rplKaJL-rpoBC operon fragments caused by an overproduction of ribosomal proteins in pUC were studied.


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