Biopolym. Cell. 1989; 5(1):41-45.
Structure and Function of Biopolymers
The protein composition of nuclear matrix in cells of different animal tissues
1Blokhin D. Yu., 1Struchkov V. A.
  1. All-Union Cancer Research Centre, Academy of Medical Sciences of the USSR
    Moscow, USSR


The protein composition of nuclear matrix preparations, isolated from different animal tissues under the same conditions, possesses species and tissue specificity. The preparations of nuclear matrix are resistant to additional treatments with dissociating agents (EDTA, NaCl, urea), but their incubation in the presence of thiols results in dissociation of some proteins, that confirms the assumption on participation of disulfide intersubunit bonds in organization of skeletal structures of interphase nucleus


[1] Zbarsky IB. Protein composition and organization of the nuclear matrix. Biopolym. Cell. 1985; 1(1):26-32.
[2] Chernokhvostov VV. The nuclear matrix of eukaryotic cells: some questions of isolation, structure and functioning. Usp sovrem biol. 1985; 99(3):371-84.
[3] Berezney R. Dynamic properties of the nuclear matrix. The cell nucleus. Ed. H. Busch. New York : Acad, press, 1979. Vol. 7: 413-456.
[4] Berezney R, Buchholtz LA. Isolation and characterization of rat liver nuclear matrices containing high molecular weight deoxyribonucleic acid. Biochemistry. 1981;20(17):4995-5002.
[5] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[6] Osterman LA. Methods of study of proteins and nucleic acids. Electrophoresis and ultracentrifugation. Moscow, Nauka, 1981; 288 p.
[7] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[8] Iarovaia OV, Razin SV. Two types of attachment points of DNA to the nuclear skeleton in Ehrlich ascites carcinoma cells. Mol Biol (Mosk). 1983;17(2):303-13.
[9] Chernokhvostov VV. Proteins tightly associated with DNA and their role in the organization of sites of attachment of DNA to the skeletal structure of chromosomes: Author. dis. ... Cand. biol. nauk. Moscow, 1985; 23 p.
[10] Douvas AS, Harrington CA, Bonner J. Major nonhistone proteins of rat liver chromatin: preliminary identification of myosin, actin, tubulin, and tropomyosin. Proc Natl Acad Sci U S A. 1975;72(10):3902-6.
[11] Nakayasu H, Ueda K. Association of actin with the nuclear matrix from bovine lymphocytes. Exp Cell Res. 1983;143(1):55-62.
[12] Wunderlich F, Herlan G. Reversibly contractile nuclear matrix. Its isolation, structure, and composition. J Cell Biol. 1977;73(2):271-8.