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Species and tissue-specific variations in distribution of mammalian tryptophanyl-tRNA synthetase: an abnormal high content in bovine pancreas
- Institute of Molecular Biology, Academy of Sciences of the USSR
- Research Institute of Experimental Cardiology, National Cardiological Research Centre, Academy of Medical Sciences of the USSR
The content of tryptophanyl-tRNA synthetase has been determined in human, bovine, rat and rabbit liver and pancreatic extracts by direct radioimmunoblotting with 125I-labelled monoclonal antibody. It was in bovine but not in other pancreas that the unusually high amount of tryptophanyl-tRNA synthetase was estimated (about 0.2 %). According to the indirect immunofluorescent method, bovine tryptophanyl-tRNA synthetase is located in exocrinic part of the pancreatic gland.
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