Biopolym. Cell. 1988; 4(6):283-289.
Structure and Function of Biopolymers
Theoretical analysis of addressed modification of DNA
1Perelroyzen M. P., 2Vologodskii A. V.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. Institute of Molecular Genetics, Academy of Sciences of the USSR
    Moscow, USSR


Specific chemical addressed modification of DNA involves treatment of single-stranded DNA by oligonucleotides complementary to certain target sequences in this DNA and bearing groupings reactive towards the DNA bases. The binding with the partially complementary targets can cause non-specific modification. The selectivity of the addressed modification is the subject of the present investigation. Modification of a fragment is analyzed which is flanked by two target sequences complementary to a given oligonucleotide address and contains no more such exact targets over all its length. Conditions of maximum ratio between specific and non-specific modification are calculated. The probability of the process in which up to the given time both of the target termini will be specifically modified without any non-specific modification of the internal part of a fragment is determined.


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