Biopolym. Cell. 2019; 35(6):476-485.
Viruses and Cell
Genetic diversity of Plum pox virus in Ukraine
1Kutsenko O. V., 1Budzanivska I. G., 1Shevchenko O. V.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601


Aim. Establishing the genetic diversity of Plum pox virus (PPV) in Ukraine. Methods. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polymerase chain reaction with reverse transcription (RT-PCR), DNA sequencing and phylogenetic analysis. Results. The samples with visual symptoms of virus infection were collected from different regions of Ukraine and diagnosed with DAS-ELISA. Subsequently, the amplicons of the coat protein (CP) gene were obtained using RT-PCR and sequenced. Obtained viral cDNAs were analyzed using phylogenetic methods. As a result, the genetic diversity of PPV in different regions of Ukraine has been elucidated. The phylogenetic relationships between Ukrainian isolates were studied with subsequent comparison to PPV isolates from neighboring countries. Conclusions. The PPV strains M and D circulate in Ukraine. For the first time, PPV was found in the Kharkiv region. The PPV isolates circulating in Ukraine are most similar to the isolates from Germany, Turkey, Hungary, and Canada. The results can be used in the future to predict the spread of the virus in different regions of Ukraine and neighboring countries, to establish its origin and predict the development of possible epidemics.
Keywords: Plum pox virus, coat protein gene, phylogenetic analysis, sequencing, genetic diversity, Ukraine


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