Biopolym. Cell. 2019; 35(4):313-320.
Expression pattern of immune- and cancer-associated genes in peripheral blood of mice bearing melanoma cells
1Gerashchenko G. V., 1Vagina I. M., 1Vagin Yu. V., 1Tkachuk Z. Yu., 1Kashuba V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143


Aim. To identify putative non-invasive expression markers, based on relative expression (RE) of cancer- and immune-associated genes, in peripheral blood of mice, bearing melanoma cells. Methods. RE of 56 cancer- and immune-associated genes was assessed byquantitative PCR in peripheral blood of C57BL/6j mice inoculated with B16 mouse melanoma cells and in control animals. Results. Eleven genes showed significant differences in the RE levels in mice,bearing melanoma: six genes (Ccl5, Il1b, Mif, Rnasel, S100a1 and Tgfb1) were expressed at higher levels, and five genes (Erbb2, Ifnb1, Il6, Pdcd1 and Prom1) were downregulated in comparison with the control animals. We have demonstrated a stable immunosuppressed state of mice inoculated with melanoma cells as evidenced by decreased RE levels of Ifnb1 and Pdcd1 and increased RE levels of Lbp, Tlr3, Tlr8, Gstp1, Prom1, Oas1a, Oas3 and Il1b. Conclusions. Assessment of expression of cancer- and immune-associated genes in peripheral blood during growth of malignant cells in experimental animals may result in discovery of effective noninvasive expression markers for the prognosis of the cancer outcome and chemotherapy efficiency
Keywords: melanoma, relative gene expression, immune-associated genes, putative expression markers.


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