Biopolym. Cell. 2019; 35(3):184-184.
Chronicle and Information
mTORC1 pathway in DNA damage response
1Ma Y., 1Silveri L., 2LaCava J., 1Vassetzky Y., 1Dokudovskaya S.
  1. CNRS UMR 8126, Université Paris-Sud, Gustave Roussy
    114, rue Edouard Vaillant, 94805, Villejuif, France
  2. Laboratory of Cellular and Structural Biology, The Rockefeller University, New York
    New York, USA.


Living organisms have evolved various mechanisms to control their metabolism and response to various stresses, allowing them to survive and grow in different environments. In eukaryotes, the highly conserved mechanistic target of rapamycin (mTOR) signaling pathway integrates both intracellular and extracellular signals and serves as a central regulator of cellular metabolism, proliferation and survival. A growing body of evidence indicates that mTOR signaling is closely related to another cellular protection mechanism, the DNA damage response (DDR). Many factors important for mTOR pathway are also involved in the DDR. The SEA/GATOR complex is an inhibitor of the mTORC1 pathway. In mammals the GATOR1 complex is composed of the proteins DEPDC5, NPRL2 and NPRL3. We characterized mammalian cells overexpressing the GATOR1 component NPRL2 and found that in the cells with active p53, ectopic expression of NPRL2 induces NOX2-dependent production of reactive oxygen species and DNA damage. Overexpressed NPRL2 accumulates in the nucleus, together with apoptosis-inducing factor (AIF). These events are accompanied by phosphorylation of p53, activation of a DNA-damage response and cell cycle arrest in G1 phase, followed by apoptosis. In the cells negative for active p53, NPRL2 ectopic expression leads to activation of CHK1 or CHK2 kinases and cell cycle arrest in S or G2/M phases. Combined, these results demonstrate a new role for the NPRL2, distinct from its function in mTORC1 regulation.