Biopolym. Cell. 2019; 35(3):178-179.
Chronicle and Information
Lamin A and PI(4,5)P2 - a novel complex in the cell nucleus
1Escudeiro-Lopes S., 1Philimonenko V. V., 1Jarolimová L., 1Hozák P.
  1. Department of Biology of the Cell Nucleus, Institute of Molecular Genetics ASCR v.v.i.
    Vídeňská 1083, 142 20, Prague 4, Czech Republic


Lamins are important regulators of nuclear structural integrity as well as nuclear functional processes such as DNA transcription, replication and repair, and epigenetic regulation. Mutations in LMNA gene, which encodes for lamin A protein, cause a large variety of human diseases, known as laminopathies, including muscular dystrophies and progeroid syndromes. Phosphatidynositol-4,5-bisphosphate (PIP2) is a well described product of the phosphoinositol signalling and it has an important role in splicing and transcription. Here we show that PIP2 is in a complex with lamin A, together with Nuclear myosin 1 (NM1), and lamin A phosphorylation status appears to be implicated in the anchoring of other proteins to the complex, as well as in the movement of lamin A from the nuclear envelope to the nucleoplasm. Thus, we are characterizing this complex using several biochemical and microscopy methods. From our preliminary data we conclude that some Lamin A phosphorylations might be important for PIP2-dependent interactions of lamin A, important for nuclear functions. Funding: We would like to thank to the Grant Agency of the Czech Republic (17-09103S, 16-03346S and 15-08738S) for their financial support to this project. We would also like to thank for the institutional support due to the long-term conceptual development of the scientific organization (RVO: 68378050). The microscopy work was performed at the Microscopy Centre, Institute of Molecular Genetics AS CR, supported by the MEYS CR (LM2015062 Czech Bioimaging).