Biopolym. Cell. 2017; 33(3):172-182.
Structure and Function of Biopolymers
Surface-exposed actin binds plasminogen on the membrane of agonist-activated platelets: a flow cytometry study
1Tykhomyrov A. A., 1Zhernossekov D. D., 1Grinenko T. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

Aim. To elucidate whether actin exposed on the surface of agonist-activated platelets is responsible for plasminogen binding. Methods. Human washed platelets were obtained by gel-filtration on Sepharose-2B. The levels of exposed actin on the outer surface of plasma membrane of intact and thrombin- or collagen-stimulated platelets were monitored by flow cytometry. The binding of plasminogen fluorescent conjugate (Pg-FITC) with platelets treated with anti-actin antibody or non-immune IgG (control) was analyzed cytometrically. Results. Thrombin or collagen exposure resulted in the dose-dependent actin expression on the surface of stimulated platelets. Pre-treatment of stimulated platelets with specific anti-actin antibodies was shown to significantly prevent the binding of Pg-FITC with the platelet surface. Conclusion. The results of the present study indicate that actin exposed on the platelet surface at the agonist-induced activation is involved in the plasminogen binding.
Keywords: platelets, surface-exposed actin, plasminogen, flow cytometry

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