Biopolym. Cell. 2017; 33(2):92-101.
Biomedicine
Single center study of ESBL-related strains of Enterobacteriaceae collected from clinical specimens of infants with the congenital heart disease using multiplex PCR amplification
1Filonenko G. V., 1Talalaiev O. S., 2Kyryk D. L., 1Kovalenko N. O., 1Skorohod I. M., 1Salamanina A. O.
  1. Center for Pediatric Cardiology and Cardiac Surgery
    28./1, Chornovola Str. Kyiv, Ukraine, 01135
  2. P. L. Shupik National medical academy of post-graduate education
    9, Dorohozhytska Str., Kyiv, Ukraine, 04112

Abstract

Aim. To study ESBL phenotypes, as well as to identify individual genotypes of β-lactamases and to study their prevalence among clinical strains of Enterobacteriaceae collected from children with the congenital heart disease. Methods. The susceptibility of clinical strains of Enterobacteriaceae to antibiotics was using an automated system; the resistance determinants were genotyped using multiplex PCR. Results. During the period of the study, 10.9 % of ESBL-positive isolates were found among clinical strains of Enterobacteriaceae. Most ESBL-producing strains (33.3 %) were Klebsiella pneumoniae. Most strains producing extended-spectrum β-lactamases were isolated from the respiratory tract and accounted for 83.3 %. Conclusions. This study provided new data on the prevalence of ESBL-producing genetic resistance determinants within the Enterobacteriaceae family and their role in the development of complications. We genotyped ESBL-positive isolates using multiplex PCR. The study demonstrated the diagnostic potential of molecular biology methods in identifying resistance determinants of microorganisms.
Keywords: antibiotic resistance, β-lactam antibiotics, Enterobacteriaceae, ESBL, PCR, genotyping

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