Biopolym. Cell. 2017; 33(1):48-57.
http://dx.doi.org/10.7124/bc.00093F
Molecular and Cell Biotechnologies
Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics
1Kiroshka V. V., 2Savvova O. V., 1Bozhkova Yu. O., 1Tamarina I. V., 2Fesenko A. I.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
  2. National Technical University “Kharkiv Polytechnic Institute”
    2, Kyrpychova str., Kharkiv, Ukraine, 61002

Abstract

Aim. To study spreading profile, cytoskeleton organization and proliferation of bone marrow stromal cells upon cultivation on the glass-crystalline material (GCM) surfaces with different chemical composition and solubility. Methods. GCMs with different CaO: P2O5 ratios were used. Actin cytoskeleton in cells was visualized using fluorescent TRITC-conjugated phalloidin. Cell proliferation was studied using MTT test. Results. Cell cultivation on highly soluble B-series GCM (Ca/P=5) led to appearance of fibroblast-like cells; their actin cytoskeleton filaments were uniformly distributed within the cytoplasm. In this case, proliferation dynamics was similar to that under cultivation on plastic. Cultivation on A and C series of GCM with a reduced Ca/P (1.4–2.58) resulted in a decrease of cell spreading and their proliferation index (up to 2–3 times) relative to the control. Conclusions. The material solubility and Ca/P superficial ratio are the main factors, determining cell interaction with GCMs.
Keywords: glass-crystalline materials, bone marrow stromal cells
Full text: (PDF, in English)

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