Biopolym. Cell. 2015; 31(3):187-192.
Structure and Function of Biopolymers
Generation and characterization of monoclonal antibodies specific to Coenzyme A
1Malanchuk O. M., 1Panasyuk G. G., 1Serbyn N. M., 2Gout I. T., 1Filonenko V. V.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Institute of Structural and Molecular Biology, University College London
Gower Street, London WC1E 6BT, UK
Abstract
Aim. Generation of monoclonal antibodies specific to Coenzyme A. Methods. Hybridoma technique. KLH carrier protein conjugated with CoA was used for immunization. Screening of positive clones was performed with BSA conjugated to CoA. Results. Monoclonal antibody that specifically recognizes CoA and CoA derivatives, but not its precursors ATP and cysteine has been generated. Conclusion. In this study, we describe for the first time the production and characterization of monoclonal antibodies against CoA. The monoclonal antibody 1F10 was shown to recognize specifically CoA in Western blotting, ELISA and immunoprecipitation. These properties make this antiboby a particularly valuable reagent for elucidating CoA function in health and disease.
Keywords: CoA, hybridoma technique, monoclonal antibody
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