Biopolym. Cell. 2014; 30(6):462-468.
http://dx.doi.org/10.7124/bc.0008C1
Molecular and Cell Biotechnologies
Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes
1, 2Pyeshkova V. N., 2Dudchenko O. Y., 1, 2Soldatkin O. O., 1, 3Kucherenko I. S., 4Ozansoy Kasap B., 4Akata Kurc B., 1, 2Dzyadevych S. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of High Technologies,
    Taras Shevchenko National University of Kyiv
    2, korp.5, Pr. Akademika Hlushkova, Kyiv, Ukraine, 03022
  3. Educational and Scientific Center "Institute of Biology",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  4. Micro and Nanotechnology Department, Middle East Technical University
    Ankara, Turkey, 06531

Abstract

Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and compared: GOx adsorption on silicalite modified electrodes (GOx-SME); cross-linking by glutaraldehyde without silicalite (GOx-GA); GOx adsorption on SME along with cross-linking by glutaraldehyde (GOx-SME-GA). The GOx-SME-GA biosensors based on stainless steel electrodes were characterized by 12–25-fold higher sensitivity comparing with other biosensors. The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. This method is found to be promising for further creation of other enzyme biosensors.
Keywords: enzyme immobilization, silicalite, glucose oxidase, conductometric transducer, biosensor
Full text: (PDF, in English)

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