Biopolym. Cell. 2014; 30(4):310-313.
Biomedicine
Association of genotypes by polymorphic variant C-108T of PON1 gene with the risk of developing breast cancer and hypertensive disease in women
1Fishchuk L. Ye.
  1. Institute of Genetic and Regenerative Medicine, NAMS of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114

Abstract

Aim. To analyze the effect of the polymorphic variant C-108T of PON1 gene on the risk of developing breast cancer (BC) and hypertensive disease (HD) in women. Methods. 130 women with non-complicated HD of degree II and 131 women with BC of I and II stages without cardiovascular complications were enrolled in the study. The control group was composed of 102 women without cardiovascular and oncological pathologies. Molecular-genetic analysis was performed on DNA isolated from the samples of peripheral blood. Genotyping of polymorphic variant C-108T of PON1 gene was carried out with the use of PCR-RFLP method. Results. It has been revealed that the presence of -108CC genotype of PON1 gene in women elevates the risk of HD development in more than 1.5 times. As for women over 54, the presence of -108CC genotype of PON1 gene is associated with a decreased risk of BC by almost 4 times and, in contrary, the presence of -108CT genotype of PON1 gene is associated with an increased risk of BC by more than 3 times. Conclusions. Polymorphic variant C-108T of PON1 gene may be considered as possible prognostic marker of BC and HD development in women.
Keywords: gene, PON1, breast cancer, hypertensive disease.

References

[1] Stocks T, Van Hemelrijck M, Manjer J, Bj?rge T, Ulmer H, Hallmans G, Lindkvist B, Selmer R, Nagel G, Tretli S, Concin H, Engeland A, Jonsson H, Stattin P. Blood pressure and risk of cancer incidence and mortality in the Metabolic Syndrome and Cancer Project. Hypertension. 2012;59(4):802-10.
[2] Li CI, Daling JR, Tang MT, Haugen KL, Porter PL, Malone KE. Use of antihypertensive medications and breast cancer risk among women aged 55 to 74 years. JAMA Intern Med. 2013;173(17):1629-37.
[3] Grdic M, Barisik K, Rumora L, Salamunic I, Tadijanovic M, Grubisic TZ, Psikalova R, Flegar-Mestric Z, Juretic D. Genetic frequencies of paraoxonase 1 gene polymorphisms in Croatian population. Croat Chem Acta. 2008;81(1):105–11.
[4] Polonikov AV, Solodilova MA, Khoroshaya IV, Katargina LN, Kozhukhov MA, Kolesnikova OE. Association study of the two-point mutations of the paraoxonase genes Q192R PON1 and S311C PON2 with predisposition to essential hypertension in Russian inhabitants of Central Chernozem region of Russia. Kurskiy scientifically-practical herald «Persons and his health». 2006;(4):57–61.
[5] Yilmaz N. Relationship between paraoxonase and homocysteine: crossroads of oxidative diseases. Arch Med Sci. 2012;8(1):138-53.
[6] Bhatnagar V, Liu L, Nievergelt CM, Richard E, Brophy VH, Pandey B, Lipkowitz MS, O'Connor DT. Paraoxonase 1 (PON1) C/T-108 association with longitudinal mean arterial blood pressure. Am J Hypertens. 2012;25(11):1188-94.
[7] Voetsch B, Benke KS, Panhuysen CI, Damasceno BP, Loscalzo J. The combined effect of paraoxonase promoter and coding region polymorphisms on the risk of arterial ischemic stroke among young adults. Arch Neurol. 2004;61(3):351-6.
[8] Searles Nielsen S, McKean-Cowdin R, Farin FM, Holly EA, Preston-Martin S, Mueller BA. Childhood brain tumors, residential insecticide exposure, and pesticide metabolism genes. Environ Health Perspect. 2010;118(1):144-9.
[9] Stevens VL, Rodriguez C, Pavluck AL, Thun MJ, Calle EE. Association of polymorphisms in the paraoxonase 1 gene with breast cancer incidence in the CPS-II Nutrition Cohort. Cancer Epidemiol Biomarkers Prev. 2006;15(6):1226-8.
[10] Antognelli C, Del Buono C, Ludovini V, Gori S, Talesa VN, Crin? L, Barberini F, Rulli A. CYP17, GSTP1, PON1 and GLO1 gene polymorphisms as risk factors for breast cancer: an Italian case-control study. BMC Cancer. 2009;9:115.
[11] Saadat M. Paraoxonase 1 genetic polymorphisms and susceptibility to breast cancer: a meta-analysis. Cancer Epidemiol. 2012;36(2):e101-3.
[12] Uzun H, Karter Y, Aydin S, Curgunlu A, Sim?ek G, Y?cel R, Vehiyd S, Ert?rk N, Kutlu A, Benian A, Yaldiran A, Ozt?rk E, Erdine S. Oxidative stress in white coat hypertension; role of paraoxonase. J Hum Hypertens. 2004;18(7):523-8.