Biopolym. Cell. 2014; 30(4):291-298.
Molecular and Cell Biotechnologies
Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation
1, 2Soldatkin O. O., 3Ozansoy Kasap B., 3, 4Akata Kurc B., 1, 2Soldatkin A. P., 1, 2Dzyadevych S. V., 1El’skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of High Technologies,
    Taras Shevchenko National University of Kyiv
    2, korp.5, Pr. Akademika Hlushkova, Kyiv, Ukraine, 03022
  3. Central Laboratory, Middle East Technical University
    Ankara, Turkey, 06531
  4. Micro and Nanotechnology Department, Middle East Technical University
    Ankara, Turkey, 06531

Abstract

Aim. Optimization of a new method of enzyme immobilization for amperometric biosensor creation. Methods. The amperometric biosensor with glucose oxidase immobilized on zeolites as bioselective elements and platinum disk electrode as transducers of biochemical signal into the electric one was used in the work. Results. The biosensors based on glucose oxidase adsorbed on zeolites were characterized by a higher sensitivity to glucose and a better inter-reproducibility. The best analytical characteristics were obtained for the biosensors based on nano beta zeolite. It has been found that an increase in the amount of zeolite on the surface of amperometric transducer may change such biosensor parameters as sensitivity to the substrate and duration of the analysis. Conclusions. The proposed method of enzyme immobilization by adsorption on zeolites is shown to be quite promising in the development of amperometric biosensors and therefore should be further investigated.
Keywords: Biosensor, amperometric transducer, enzyme adsorption, silicalite, nano beta zeolite, glucose oxidase

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