Biopolym. Cell. 2014; 30(2):135-140.
Molecular and Cell Biotechnologies
Adhesion and proliferation of adipose derived mesenchymal stromal cells on chitosan scaffolds with different degree of deacetylation
1Rogulska O. Yu., 1Petrenko Yu. O., 2Kalinkevich O. V., 2Kalinkevich A. N., 1Petrenko O. Yu.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
  2. Institute of Applied Physics, NAS of Ukraine
    58, Petropavlivska Str., Sumy, Ukraine, 40030


Aim. Selection of the optimal scaffold for the creation of tissue engineering constructs is a key challenge of biotechnology. In this study we investigated the biocompatibility of human adipose derived mesenchymal stromal cells (MSCs) within the three-dimensional matrices based on the chitosan with a different degree of deacetylation. Methods. MSCs were seeded on the chitosan scaffolds by a perfusion method and cultured for 7 days. The morphology, viability, metabolic activity and distribution of the cells within the matrices were analyzed. Results. The level of MSCs adhesion to the surface of the chitosan scaffolds with low degree of deacetylation (67 %) was insignificant, the cells were round and formed aggregates. In the chitosan scaffolds with a high degree of deacetylation (82 %) the cells attached to the surface of matrices, were able to spread and proliferate. Conclusions. The chitosan scaffolds with a high degree of deacetylation and the human adipose derived MSCs can be used for the creation of bioengineered structures.
Keywords: chitosan scaffolds, adipose derived mesenchymal stromal cells, tissue engineering, degree of deacetylation


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