Biopolym. Cell. 2014; 30(2):129-134.
Biomedicine
Comparative analysis of epigenetic markers in plasma and tissue of patients with colorectal cancer
1Kondratov A. G., 1Nekrasov K. A., 1Lototska L. V., 1Panasenko G. V., 1Stoliar L. A., 1Lapska Y. V., 2Kolesnyk O. O., 2Shchepotin I. B., 1Rynditch A. V., 1, 3Kashuba V. I.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Cancer Institute
    33/43, Lomonosova Str., Kyiv, Ukraine, 03022
  3. Department of Microbiology, Tumor and Cell Biology,
    Karolinska Institute
    Nobels vag 5 171 65 Solna, Sweden

Abstract

Aim. The work is devoted to the development of less invasive tools for the colorectal cancer (CRC) screening. Methods. Q-PCR and methylation-specific PCR techniques were used in the current work. Results. We have shown that the levels of cell-free plasma DNA are higher in the CRC patients compared with the healthy donors (p < 0.01). Hypermethylation of APC, FHIT, LRRC3B and HIC1 genes was studied in the tumor and plasma samples of CRC patients. Two-stage verification for CRC screening was proposed. Conclusions. We proposed and tested a novel approach for CRC screening based on the determination of cell-free DNA and methylated DNA fragments in the plasma.
Keywords: colorectal cancer, cell-free DNA, DNA methylation, APC, FHIT, LRRC3B , HIC1

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