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A simple method for isolation of haploid protoplasts from forms of rice multishoots
- Institute of Experimental Biology
Ho Chi Minh City, Vietnam
One-month old sterile plantlets grown on the 0.3 M sucrose medium were used for protoplast isolation. The young part of leaves were cut onto small pices (1 mm) and left for over night incubation in the 2 % v/v driselase solution containing glucose 0.4 M. Isolated protoplasts were purified by two-phase centrifugation (the upper phase contains solution of salts, the lower one вЂ” 0.4 M of sucrose and other nutritional constituents). The purified protoplasts were suspended between two phases.
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