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Short Communications
A simple method for isolation of haploid protoplasts from forms of rice multishoots
1Huynh Xuan Thao, 1Trinh Manh Dung, 1Ngo Ke Suong
  1. Institute of Experimental Biology
    Ho Chi Minh City, Vietnam


One-month old sterile plantlets grown on the 0.3 M sucrose medium were used for protoplast isolation. The young part of leaves were cut onto small pices (1 mm) and left for over night incubation in the 2 % v/v driselase solution containing glucose 0.4 M. Isolated protoplasts were purified by two-phase centrifugation (the upper phase contains solution of salts, the lower one — 0.4 M of sucrose and other nutritional constituents). The purified protoplasts were suspended between two phases.


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