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Vectors for cloning and activity determination of the elements of the bacterial transcription system: cloning the transcription terminator of the rRNA operon of the archaebacterium Halobacterium halobium
- M. V. Lomonosov State University
- D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR
The pBR322 vector was used for constructing recombinant plasmids pMM24 and pVR41, containing the promoter region PR of the bacteriophage λ including the transcription and translation starting points of the cro-repressor, the polylinker with the restriction sites for several restrictases and the tandem of the terminators of the fd phage. The constructed mini-gene is transcribed in E. coli cells into the corresponding mini-mRNA. The transcription of this gene could occur either constitutively or under the conduction of the c1857 represser of the phage λ. The polylinker sequence in the gene body could be used to introduce various regulator structures between the promoter and the terminators. A putative transcription terminator of the rRNA operon from the archaebacterium H. halobium was cloned inside the mini-gene. This archaebacterial sequence was shown to function as an effective terminator in the eubacterial system.
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