Biopolym. Cell. 1987; 3(3):163-166.
Short Communications
Isolation of individual proteins from 50S subparticles of Thermus thermophilus ribosomes. crystallization of TL7 protein
1Sedelnikova S. E.
  1. Institute of Protein Research, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

A method of selective extraction is suggested for eight proteins of 50S subparticles of ribosomes from extremal-thermophilic bacterium Thermus thermophilus at 60 °C in the presence of 0.5 M and 1 M NH4C1 and 50 % ethanol. As a result of chromatographic separation of extracted proteins on CM-sepharose CL five individual proteins (the purity is not less than 95 %) have been obtained. One of them, TL7 (probably, an analog of L6 protein from E. coli ribosomes), has been crystallized by the method of «hanging» drop with ammonium sulfate as a precipitant.

References

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