Biopolym. Cell. 2013; 29(6):511-514.
http://dx.doi.org/10.7124/bc.000842
Bioorganic Chemistry
Lepidine Orange derivative as a new dye for sensitive fluorescent detection of DNA
1Didan Yu. V., 2Kryvorotenko D. V., 2Negrutska V. V., 2Dubey I. Ya.
  1. Educational and Scientific Center "Institute of Biology",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. To study new cyanine dye LO-7 as a reagent for dsDNA visualization in electrophoretic gels. Methods. Gel electrophoresis, restriction, fluorescence detection, mobility shift assay. Results. LO-7 binds to DNA to form stable highly fluorescent complexes. As little as 80 pg DNA can be detected in LO-7-stained agarose gel using a laser scanner, and 0.3 ng with UV-transilluminator. This sensitivity is several times higher than can be achieved with ethidium bromide, and close to that of SYBR Green I. Conclusions. LO-7 belongs to the most efficient stains for dsDNA visualization and thus can be used in bioanalytical applications where high sensitivity is required.
Keywords: fluorescent dyes, cyanines, gel electrophoresis, nucleic acids, detection
Full text: (PDF, in English)

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