Biopolym. Cell. 2012; 28(2):149-155 .
Genomics, Transcriptomics and Proteomics
Alleles of Ppd-D1 gene in the collection of Aegilops tauschii accessions and bread wheat varieties
1Chebotar G. O., 1, 2Chebotar S. V., 2Babenko D. O., 3Motsnyy I. I., 4Scherban A. B., 1Sivolap Yu. M.
- South Plant Biotechnology Center, NAAS of Ukraine
3, Ovidiopolska doroga, Odesa, Ukraine, 65036 - Odesa I. I. Mechnikov National University
2, Dvoryanska Str., Odesa, Ukraine, 65082 - Plant breeding and Genetics Institute, National Centre of Seed and Cultivar Investigation
Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036 - The Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences
10, Prosp. Akad. Lavrentyeva, Novosibirsk, Russian Federation, 630090
Abstract
Light period significantly influences on the growth and development of plants. One of the major genes of photoperiod sensitivity
is Ppd-D1, located on the chromosome 2D. The aim of the work was to determine the alleles and molecular structure of Ppd-D1
gene in samples from the collection of Ae. tauschii accessions, which have different flowering periods,
and in 29 Ukrainian wheat varieties. Methods. We used methods of allele-specific PCR with primers to the Ppd-D1
gene, sequencing and Blast-analysis. Results. The collection of Ae. tauschii accessions and several
varieties of winter and spring wheat was studied. The molecular structure of the allelic variants
(414, 429 and 453 b. p.) of Ppd-D1b gene was determined in the collection of Aegilops. tauschii accessions.
Conclusions. The Ppd-D1a allele was present in all studied varieties of winter wheat. 60 % of spring wheat is
characterized by Ppd-D1b allele (size of amplification products 414 b. p.). Blast-analysis of the sequence data
banks on the basis of the reference sequence of sample k-1322 from the collection of Ae. tauschii accessions
has shown a high homology (80 to 100 %) between the nucleotide sequences of PRR genes, that characterize the A and D
genomes of representatives of the genera Triticum and Aegilops.
Keywords: Aegilops tauschii, bread wheat, allele-specific PCR, phd-D1
Full text: (PDF, in Ukrainian)
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