Biopolym. Cell. 2010; 26(2):153-159.
Genomics, Transcriptomics and Proteomics
RNA interference of genes of ubiquitn and proteasome β7 subunit
in cardiomyocytes culture
1Kyrychenko V. O., 1Nagibin V. S., 1Tumanovska L. V., 2Rybalchenko V. K., 1Dosenko V. E., 1Moibenko O. O.
- Bogomoletz Institute of Physiology, NAS of Ukraine
4, Bogomolets Str., Kyiv, Ukraine, 01024 - Taras Shevchenko National University of Kyiv
64, Volodymyrska Str., Kyiv, Ukraine, 01601
Abstract
Aim. Ubiquitin (UBB) and proteasome 7 subunit (PSMB7) genes silencing is interesting due to the crucial role of ubiquitin-dependent proteasomal system (UPS) in turnover of functional proteins, which controls program cell death (apoptosis, auophagy). Methods. We used methods of RNA interference (for UBB and PSMB7 genes silencing), fluorescence microscopy and real-time PCR. Results. It was shown that small interference RNA injection in cell culture decreased ubiquitin expression in 2,4 (P < < 0.05), PSMB7 – in 1,6 times (P > 0.05). At the same time, there was augmented level of necrotic cells with no change in number of apoptotic cells. Cells with signs of autophagy were much augmented as consequence of UPS impairment and intracellular proteins accumulation, which degrade in lysosomes. Conclusions. The data obtained testify that UBB and PSMB7 genes silencing induces cell necrosis and autophagy without changing number of apoptotic cells.
Keywords: ubiquitin, proteasome, RNA-interference, cardiomyocytes
Full text: (PDF, in Ukrainian)
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