Biopolym. Cell. 2009; 25(3):204-209.
Molecular and Cell Biotechnologies
Application of enzyme multibiosensor for toxicity analysis of real water samples of different origin
1Soldatkin O. O., 2Pavluchenko O. S., 2Kukla O. L., 1, 3Kucherenko I. S., 1, 3Peshkova V. M., 1Arkhypova V. M., 1Dzyadevych S. V., 1Soldatkin A. P., 1El'skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. V. Ye. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine
    41, Prospect Nauki, Kyiv, Ukraine, 03028
  3. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601


Aim. The analysis of toxicity of different water samples with the multibiosensor developed earlier. Methods. The potentiometric multibiosensor with several immobilized enzymes as bioselective elements and the matrix of pH-sensitive field effect transistors as transducers of the biochemical signal into the electric one was applied for the analysis. Results. The bioselective elements of the multibiosensor were developed using acetylcholinesterase, butyryl-cholinesterase, urease, glucose oxidase, and three-enzyme system (invertase, mutarotase, glucose oxidase). The measurement of toxic compounds in water samples of different origin was performed using the constructed sensor. The results obtained were compared with those obtained by the conventional methods of toxic agent’s analysis (atomic absorption spectrometry, thin-film chromatography, and atomic absorbic analyser of mercury). Conclusion. A strong conformity between the results obtained with the multibiosensor and traditional methods has been shown.
Keywords: multibiosensor, pH-sensitive field-effect transistors, enzymes, inhibitory analysis, pesticide, ions of heavy metal, toxins


[1] Coulet P. R. What is a biosensor Biosensor principles and application. Eds L. J. Blum, P. R. Coulet New York: Marcel Dekker, 1991:. 1–6.
[2] Thevenot D. R., Toth K., Durst R. A., Wilson G. S. Electrochemical biosensors: recommended definitions and classification (Technical report). Pure Appl. Chem. 1999; 71:2333–2348.
[3] Hall E. A. H. Recent progress in biosensor development Int. J. Biochem 1988 20, N 4:. 357–362.
[4] Scheller F. W., Pfeiffer D. Commercial Devices based on amperometric biosensors Handbook of biosensors and electronic noses: medicine, food, and environment. Ed. E. Kress-Rogers New York: CRC press, 1997:. 245–256.
[5] Soldatkin O. O., Pavluchenko O. S., Kukla O. L., Arkhipova V. M., Dzyadevych S. V., Soldatkin O. P., El'skaya A. V. Optimization of enzymatic bioselective elements as components of potentiometric multibiosensor Biopolym. Cell 2008 24, N 1:. 42–50.
[6] Soldatkin O. O., Pavluchenko O. S., Kukla O. L., Arkhipova V. M., Dzyadevych S. V., Soldatkin O. P., El'skaya A. V. Optimization of multibiosensor operation for inhibitory analysis of toxins Biopolym. Cell 2008 24, N 6:. 494– 502.
[7] Sherma J., Zweig G. Pesticides Anal. Chem 1983 55:. 57.
[8] Tran-Minh C., Pandey P. C., Kumaran S. Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase. Biosens Bioelectron. 1990;5(6):461-71.
[9] Metodicheskie ukazaniya po opredeleniyu pestizidov v vode, produktach, kormach i tabachnych izdeliyach metodom chromotografii v tonkom sloe N 2142-80 ot 28.01.1980.