Biopolym. Cell. 2009; 25(1):56-61.
Molecular Biomedicine
Photoactive immunoconjugate of hematoporphyrin with antibodies to vascular endothelial growth factor: preparation, physicochemical and biological properties
1Lisniak I. O., 1Novichenko N. L., 1Mamchur A. A., 1Gamaliya M. F.
- R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
45, Vasilkivska Str., Kyiv, Ukraine, 01022
Abstract
Preparation of photoimmunoconjugates on the basis of hematoporphyrin and antibodies to the vascular endothelial growth factor (VEGF) is described. The obtained conjugate preserved physicochemical and biological properties of both protein component and porphyrin part – hematoporphyrin. It was shown that accumulation of the photoimmunoconjugate with VEGF antibodies in chicken embryo membranes was 2,5 times larger at the period of the neovascularization than at the stabilization.
Keywords: photodynamic therapy, angiogenesis, conjugates, hematoporphyrin, VEGF
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