Biopolym. Cell. 2008; 24(6):476-486.
Molecular and Cell Biotechnologies
Circadian rhythms of cell population structure of Rauwolfia serpentina Benth. under different culture conditions in vitro
1Parnikoza I. Yu., 2Miriuta N. Yu., 2Adonin V. I., 2Kunakh V. A.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A thermodynamic approach has been used to study dependence between the incidence of cells with various DNA content in nucleus and the proliferation rate (mitoses and amitoses). These biological fluxes and forces have been analyzed at the circadian hierarchic level upon changes in the culture conditions for the Rauwolfia serpentina K-27 strain, a producent of the anti-arrhythmic alkaloid ajmaline.
Keywords: plant tissue culture, Rauwolfia serpentina, сircadian cell, population rhythm in vitro


[1] Lee M. The chromosomal basis of somaclonal variation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 1988; 39(1):413–437.
[2] Kunakh VA Biotechnology of medicinal plants. Genetic and physiological and biochemical basis. Kyiv: Logos, 2005; 730 p.
[3] Zotin A. I., Zotina R. S. thermodynamic approach to the problems of development, growth and aging. Zhurn. obshch. Biologii. 1969; 30(1):94–109.
[4] Zotina R. S., Zotin A The combined growth equation. Zhurn. obshch. Biologii 1973; 34(4):606–616.
[5] Miryuta NY, Parnikoza IY, Al Ammuri Yu Kunakh VA. Application thermodynamic approach for studying the dynamics of cell populations in vitro using tissue culture Rauwolfia serpentina Benth producer indoline alkaloids. Biotechnology. 2006; (20):78–95.
[6] Parnikoza I. Yu., Miriuta N. Yu., Al-Ammouri Yu., Kunakh V. A. Rauwolfia serpentina Benth. cell populations dynamics in the context of different cultivation conditions in vitro Biopolym. Cell. 2008; 24(4):300-309
[7] Ripperger J. A., Shearman L. P., Reppert S. M., Schibler U. Clock, an essential pacemarker component, controls expression of circadian transcription factor DBP Genes and Develop 2000 14:679–689.
[8] Chaly AV. nonequilibrium processes in physics and biology. Kiev Nauk. dumka, 1997 183 p.
[9] Bianchi M. M. Collective behavior in gene regulation: Metabolic clocks and cross-talking FEBS J 2008 275 P. 2356–2363.
[10] Palumbo M. C., Farina L., De Santis A., Giuliani A., Colosimo A., Morelli G., Ruberti I. Collective behavior in gene regulation: Post-transcriptional regulation and the temporal compartmentalization of cellular cycles FEBS J 2008 275:2364–2371.
[11] Klevecz R. R., Li C. M., Marcus I., Frankel P. H. Collective behavior in gene regulation: The cell is an oscillator, the cell cycle a developmental process FEBS J 2008 275 P. 2372–2384.
[12] Kunakh V. A., Al-Ammouri J., Miryuta N. Ju., Mozhylevska L. P. The indoline alkaloids accumulation by Rauwolfia serpentina cell lines upon surface and submerged maintenance Biopolym. Cell. 2006; 22(2):149-156
[13] Kauhova IE, Vollosovich AG, Tsigankov VA. Select a medium for deep tissue culture Rauwolfia serpentina (Rauwolfia serpentina Benth.). Rastit. Resursy. 1981; 17(2):217–224.
[14] Kunakh V. A., Mozhilevskaya L. P., Gubar' S. I. Peculiarities of productivity and suspension clones Rauwolfia serpentina Rauwolfia serpentina Benth. in vitro. Biotekhnologiya. 2001; (4):9–21.
[15] Deklaratsiynyy pat. Ukrayiny N 12083/p1 vid 27.03.2006, Sposib vyroshchuvannya kalyusnoyi kul'tury tkanyn rauvol?fiyi zmiyinoyi Rauwolfia serpentina Benth produtsenta aymalinu. V. A. Kunakh, YU. Al'-Ammuri, L. P. Mozhylevs'ka, N. YU. Miryuta Byul. N 5.
[16] Kiernon J. A. Histological and histochemical methods. Theory and practice. New-York: Pergamon press, 1990; 364 p.
[17] Parnikoza I. YU., Miryuta N. YU., Al-Amuri YU., Adonin V. I., Kunakh V. A. Peculiarities of proliferation and differentiation processes in Rauwolfia serpentina Benth tissue culture. The Bulletin of Vavilov Society of Geneticists and Breeders of Ukraine. 2006; 4(2):210–216.
[18] Pollard J. Handbook of Computational Methods statistics Moscow: Finance and Statistics, 1982 344 p.
[19] Pechurkin N. S., Bril'kov A. V., Marchenkova T. V. Population aspects of biotechnology, Nauka, Novosibirsk, 1990; 170 p.
[20] B. Goodwin Analytical physiology of cells and developing organisms. M. Mir. 1979; 287p.