Biopolym. Cell. 2008; 24(1):28-34.
Cell Biology
Induction of apoptosis and necrosis in leukemic cells by purified IgG of blood serum of mice which were fed with cattle brain for a long time
1Kozak M. R., 1Vlizlo V. V., 2Kit Y. Y., 2Stoika R. S.
  1. Institute of Animal Biology, NAAS of Ukraine
    38, Stusa Str., Lviv, Ukraine, 79034
  2. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005


IgG preparations of mice under study were tested for their ability to induce death of cells of L1210 line of murine leukemia. The highest level of cytotoxic activity was found at affecting cells with IgG of mice that were additionally fed with cattle brain. It was shown that the test cells were dying by both apoptosis (typical signs are destruction of DNA, revealed by the method of DNA-comets, condensation and fragmentation of chromatin, discovered by fluorescent microscopy of cells, stained with Hoechst 33342) and necrosis. The latter was estimated taking into account the level of lactate dehydrogenase activity, which appeared in cultural medium of L1210 cells, and by light microscopy after cell staining according to Romanovsky-Gimza.
Keywords: ration, immunoglobulins, cytotoxic activity, apoptosis, necrosis


[1] Axelrad J. An autoimmune response causes transmissible spongiform encephalopathies. Med Hypotheses. 1998;50(3):259-64.
[2] Engelstein R, Grigoriadis N, Greig NH, Ovadia H, Gabizon R. Inhibition of P53-related apoptosis had no effect on PrP(Sc) accumulation and prion disease incubation time. Neurobiol Dis. 2005;18(2):282-5.
[3] Giese A, Kretzschmar HA. Prion-induced neuronal damage--the mechanisms of neuronal destruction in the subacute spongiform encephalopathies. Curr Top Microbiol Immunol. 2001;253:203-17.
[4] Castilla J, Hetz C, Soto C. Molecular mechanisms of neurotoxicity of pathological prion protein. Curr Mol Med. 2004;4(4):397-403.
[5] Kuwata K, Matumoto T, Cheng H, Nagayama K, James TL, Roder H. NMR-detected hydrogen exchange and molecular dynamics simulations provide structural insight into fibril formation of prion protein fragment 106-126. Proc Natl Acad Sci U S A. 2003;100(25):14790-5.
[6] Liberski PP, Gajdusek DC, Brown P. How do neurons degenerate in prion diseases or transmissible spongiform encephalopathies (TSEs): neuronal autophagy revisited. Acta Neurobiol Exp (Wars). 2002;62(3):141-7.
[7] Marella M, Chabry J. Neurons and astrocytes respond to prion infection by inducing microglia recruitment. J Neurosci. 2004;24(3):620-7.
[8] Andrievskaia O, McRae H, Elmgren C, Huang H, Balachandran A, Nielsen K. Generation of antibodies against bovine recombinant prion protein in various strains of mice. Clin Vaccine Immunol. 2006;13(1):98-105.
[9] Zhu BT. Human and animal spongiform encephalopathies are the result of chronic autoimmune attack in the CNS: a novel medical theory supported by overwhelming experimental evidence. Histol Histopathol. 2005;20(2):575-92.
[10] Ebringer A, Rashid T, Jawad N, Wilson C, Thompson EJ, Ettelaie C. From rabies to transmissible spongiform encephalopathies: an immune-mediated microbial trigger involving molecular mimicry could be the answer.
[11] Paraf A. An immunological approach to prion diseases. Med. Hypoth. 1998. 50:85–90.
[12] Lim PL, Zouali M. Pathogenic autoantibodies: emerging insights into tissue injury. Immunol Lett. 2006;103(1):17-26.
[13] Gabibov AG, Ponomarenko NA, Tretyak EB, Paltsev MA, Suchkov SV. Catalytic autoantibodies in clinical autoimmunity and modern medicine. Autoimmun Rev. 2006;5(5):324-30.
[14] Immunological methods. ed. G. Friemel. M.:Meditsina, 1987. 390p.
[15] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[16] Microscopic technique. Ed. D. S. Sarkisova, YU. L. Petrova. M.: Meditsina, 1996. 544p
[17] Chen QQ, Liu WL, Guo X, Li YJ, Guo ZG. Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Acta Pharmacol Sin. 2007;28(3):353-8.
[18] Rukovodstvo po laboratornym metodam issledovaniya. Ed. L. G. Smirnovoy, Ye. A. Kost. M.: Medgiz, 1950. 23 p.
[19] Kamins'kyi VO, Lutsyk MD, Stoika RS. Comet assay of DNA fragmentation: modification of silver staining for obtaining permanent preparations. Ukr Biokhim Zh. 2005;77(6):105-8.
[20] Mori S, Watanabe W, Shigeta S. A colorimetric LDH assay for the titration of infectivity and the evaluation of anti-viral activity against ortho- and paramyxoviruses. Tohoku J Exp Med. 1995;177(4):315-25.
[21] Filchenkov O., Stoika RS Apoptosis and cancer. Ternopil: Ukrmedknyha, 2006. 523 p.
[22] Wiegele G, Brandis M, Zimmerhackl LB. Apoptosis and necrosis during ischaemia in renal tubular cells (LLC-PK1 and MDCK). Nephrol Dial Transplant. 1998;13(5):1158-67.
[23] Poletaev AB. The Immunological Homunculus (Immunculus) in Normal State and Pathology. Biochemistry (Mosc). 2002;67(5):600-8.
[24] Poletayev A. B., Alferova V. V., Abrosimova A. A., Komissarova I. A., Sokolov M. A., Gusev Ye. I. Natural neurotropic autoantibodies and pathology of the nervous system. Neuroimmunology. 2003. 1:11–17.