Biopolym. Cell. 2007; 23(6):511-518.
Molecular and Cell Biotechnologies
Conductometric biosensor based on whole-cell microalgae for assessment of heavy metals in wastewater
1, 2, 4Berezhetskyy A. L., 2Durrieu C., 3Nguyen-Ngoc H., 4Chovelon J.-M., 1Dzyadevych S. V., 5Tran-Minh C.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Ecole Nationale des Travaux Publics de l'Etat, Laboratoire des Sciences de l'Environnement
    Vaulx-en-Velin, France
  3. University of Technology HCM
    268 rue Ly Thuong Kiet, Ho Chi Minh, Vietnam
  4. Universite Claude Bernard Lyon 1, IRCELYON UMR-CNRS 5256, Institut de Recherches sur la Catalyse et l'Environnement de Lyon
    Villeurbanne, France
  5. Ecole Nationale Superieure des Mines de Saint-Etienne
    Saint-Etienne, France


Whole-cell Chlorella vulgaris conductometric biosensors consisting of gold planar interdigitated electrodes and sol-gel algal membranes have been used for assessment of heavy-metal ions in water. These analytes act as algal alkaline phosphatase inhibitors. Enzyme residual activity has been measured in Tris-nitrate buffer in the presence of Mg2+ ions as activator. Operating conditions of this biosensor have been optimized and its characteristics are discussed. Detection limits are about 1 ppb for Cd2+, Co2+, Ni2+, Pb2+ and 10 ppb for Zn2+. The storage stability of the biosensor in buffer solution at 4 oC is more than 40 days. The biosensor has been used to assess wastewater pollution.
Keywords: Chlorella vulgaris, thin-film planar interdigitated electrodes, sol-gel immobilization, inhibitor analysis


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