Biopolym. Cell. 2006; 22(2):149-156.
Molecular and Cell Biotechnologies
The indoline alkaloids accumulation by Rauwolfia serpentina cell lines upon surface and submerged maintenance
1Kunakh V. A., 1Al-Ammouri J., 1Miryuta N. Ju., 1Mozhylevska L. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Indoline alkaloids accumulation, including ajmaline, in the biomass of R. serpentina cell line culture on the example of hormone independent highly productive strain K-27 at its surface and submerged maintenance in nutrient media with differing mineral composition has been studied. Optimal nutrient media for both ways of maintenance have been identified. The conditions for two-step maintenance in surface, followed by submerged cultures, in compositionally simple nutrient media have been specified. Two-step maintenance increases alkaloids accumulation at the early stages of growth 3–4 times and allows decreasing the time of callus tissue growing from 60–80 days to 20–40 days.
Keywords: ajmaline, indoline alkaloids, plant tissue culture, Rauwolfia serpen­tina, cell lines — alkaloids producents


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