Biopolym. Cell. 2004; 20(6):524-529.
Molecular and Cell Biotechnologies
The modeling of the growth curve of the Escherichia coli strain produsing the recombinant β-galactosidase protein
- Scientific Industrial Enterprise, Joint-Stock Company "Diaproph-Med"
35, Svetlitsky str., Kyiv, Ukraine, 04123
A kinetics analysis method of growth curves based on the Verhulst logistic function has been used to determine the some growth parameters describing physiological activity of an E. coli strain expressing a recombinant β-galactosidase protein controlled by C1857 gene. The several growth points critical for microbial population development such as transition of increasing growth speed to the decreasing one (the inflection point of the curve вЂ“ Te), the maximal growth acceleration phase (the point T1), the negative growth acceleration (slowing) phase (the point T2) have been calculated. This approach allows the prediction of the optimal conditions for the cloned gene expression in any age of the culture growth.
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