Biopolym. Cell. 2004; 20(6):505-510.
Genome and Its Regulation
Biological activity of some proteins and their usage for modulation of induced mutagenesis in mammalian somatic cells in vitro
1Macewicz L. L., 1Suhorada O. M., 1Lukash L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The influence of some proteins, such as elderberry bark and inflorescences lectins, and insulin, on cell culture proliferation, was compared. The antimutagenic properties for one of those proteins (elderberry bark lectin) were demonstrated.


[1] Lis H, Sharon N. Lectins: Carbohydrate-specific proteins that mediate cellular recognition. Chem Rev. 1998; 98 (2):637-74.
[2] Battelli MG, Citores L, Buonamici L, Ferreras JM, De Benito FM, Stirpe F, Girbes T. Toxicity and cytotoxicity of nigrin b, a two-chain ribosome-inactivating protein from Sambucus nigra: Comparison with ricin. Arch Toxicol. 1997; 71 (6):360-4.
[3] Holikova Z, Hrdlickova-Cela E, Plz?k J, Smetana K Jr, Betka J, Dvorankova B, Esner M, Wasano K, Andra S, Kaltner H, Motlik J, Hercogova J, Kodet R, Gabius HJ. Defining the glycophenotype of squamous epithelia using plant and mammalian lectins. Differentiation-dependent expression of alpha2,6- and alpha2,3-linked N-acetylneuraminic acid in squamous epithelia and carcinomas, and its differential effect on binding of the endogenous lectins galectins-1 and -3. APMIS. 2002; 110 (12):845-56.
[4] Rossner P, Bencko V, Sram R. Combined action of chromium and nickel on mouse and hamster fibroblast cell lones. J Hyg Epidemiol Microbiol Immunol. 1981;25(3):252-8.
[5] Environmental health criteria. Geneva, WHO. 1991; Vol. 108, 383 p.
[6] Chen C-Y, Wang Y-F, Lin Y-H, Yen S-F. Nickel-induced oxidative stress and effect of antioxidants in human lymphocytes. Arch Toxicol. 2003; 77 (3):123-30.
[7] Deng C, Lee HH, Xian H, Yao M, Huang J, Ou B. Chromosomal aberrations and sister chromatid exchanges of peripheral blood lymphocytes in Chinese electroplating workers: Effect of nickel and chromium. J Trace Elements Exp Med. 1988; 1 (1):57-62.
[8] Hygienic criteria of the environment. Geneva, WHO, 1991. Vol. 62. 138 p.
[9] Sen P, Costa M. Induction of chromosomal damage in Chinese hamster ovary cells by soluble and particulate nickel compounds: Preferential fragmentation of the heterochromatic long arm of the X-chromosome by carcinogenic crystalline NiS particles. Cancer Res. 1985; 45 (5):2320-5.
[10] Newman SM, Summitt RL, Nunez LJ. Incidence of nickel-induced sister-chromatid exchange. Mutat Res. 1982;101(1):67-75.
[11] Kiseleva AA, Pylev LH, Lipatov GYa. Dependence of mutagenic activity of nickel dust on the dose and time in the micronucleus test. Hygiene and sanitation. 1990; 9:71-72.
[12] Danadevi K, Rozati R, Banu BS, Grover P. Genotoxic evaluation of welders occupationally exposed to chromium and nickel using the Comet and micronucleus assays. Mutagenesis. 2004; 19 (1):35-41.
[13] Morita H, Kada K, Umeda M. Mutagenicities of nickel and cobalt compounds in mammalian cell line. Mutat Res. 1985; 147 (5):265-6.
[14] Amacher DE, Paillet SC. Induction of trifluorothymidine-resistant mutants by metal ions in L5178Y/TK+/- cells. Mutat Res/Genet Toxicol. 1980; 78 (3):279-88.
[15] Ohshima S. Induction of genetic instability and chromosomal instability by nickel sulfate in V79 Chinese hamster cells. Mutagenesis. 2003; 18 (2):133-7.
[16] Matsevych LL, Lukash LL, Sukhorada OM. Using comet assay for the evaluation of biological activity of heavy metal salts in eucaryotic cells. Ukr Biokhim Zh. 2003;75(6):75-80.
[17] Thierse HJ, Moulon C, Allespach Y, Zimmermann B, Doetze A, Kuppig S, Wild D, Herberg F, Weltzien HU. Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. J Immunol. 2004; 172 (3):1926-34.
[18] Robison SH, Costa M. The induction of DNA strand breakage by nickel compounds in cultured Chinese hamster ovary cells. Cancer Lett. 1982; 15 (1):35-40.
[19] Sjakste TG, Sjakste NI. Chemical compounds that damage DNA. Zinatne, Riga, 1991; 152 p.
[20] Wedrychowski A, Schmidt WN, Hnilica LS. The in vivo cross-linking of proteins and DNA by heavy metals. J Biol Chem. 1986; 261 (7):3370-6.
[21] Tallkvist J, Bowlus CL, Linnerdal B. Effect of iron treatment on nickel absorption and gene expression of the Divalent Metal Transporter (DMT1) by human intestinal Caco-2 cells. Pharmacol Toxicol. 2003; 92 (3):121-4.
[22] Matsevych LI, Kovalenko OA, Sukhorada OM, Lukash LL. Investigation of the influence of proteins on the genetic variability of cell populations in vitro. Factors Experimental evolution of organisms: Coll. Science, Labor. Ed. MV Royik. Kyiv, Agricultural Science, 2003:91-97.
[23] Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun. 1984; 123 (1):291-8.
[24] Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175 (1):184-91.
[25] Solovyan VT, Andreev IO, Kolotova TY, Pogrebnoy PV, Tarnavsky DV. The ordered disintegration of nuclear DNA as a specific genome reaction accompanying apoptosis, stress response and differentiation. Biopolym Cell. 1996; 12 (3):67-76.
[26] Lukash LL, Karpova IS, Miroshnichenko OS, Tikhonova TN, Lylo VV, Man'ko VG, Sukhorada EM, Golynskaia EL. The effect of the lectin from Sambucus nigra inflorescences on spontaneous and alkylating agent-induced mutagenesis in mammalian somatic cells. Tsitol Genet. 1997; 31(5):52-60.