Biopolym. Cell. 2004; 20(4):351-354.
The target antigen identification at dilated cardiomyopathy
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- Petr Bogach Institute of Physiology
Taras Shevchenko National University of Kyiv
2 corp 12, Academika Glushkova Ave Str., Kyiv, Ukraine, 03022
- M. D. Strazhesko Institute of Cardiology, MAS of Ukraine
5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
Identification of target antigen at autoimmune pathologies is based on the use of immunological approaches such as ELISA and Western blot analysis. To determine the antigen at dilated cardiomyopathy (DCM) which is a severe autoimmune cardiospecific pathology of unknown origin we have studied the level of specific antibodies against myosin. The panel of blood sera from healthy donors and DCM patients has been screened in ELISA using as antigen myosins purified from the myocardia of healthy donors and DCM patients. We have observed that the level of antimyosin antibodies in sera of DCM patients is significantly higher then in sera of healthy donors. The comparative analysis of immune response against heavy and light chains of myosins revealed that heavy chain of DCM myosin were the most immunogenic. For the first time we have demonstrated that heavy chain of DCM myosin is a target antigen at dilated cardiomyopathy.
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