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Inhibitory effect of 6-azacytidine on human cytomegalovirus infection in cellular system
- Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
5, Amosova Str., Kyiv, Ukraine, 03038
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences
16, Gamalei Str., Moscow, Russian Federation
Effects of a modified nucleoside analog 6-Azacytidine (2-b-D-ribofuranosyl-5-amino-1,2,4-triazin-3(2H)-one) on cytomegalovirus (CMV) infection in vitro, have been studied. 6-AZC inhibits the CMV cytopathic action, the effective dose of 6-AZC being 0.005 mg/ml. The analysis of cell viability, growth characteristics, and DNA synthesis has revealed that the cytotoxic effect of 6-AZC in human diploid fibroblasts is negligible. The cytotoxicity index range is 2.4 to 24 mg/ml when tested by different methods. The selectivity index of 6-AZC is 48-480, which differs negligibly from that of gancyclovir (100-1000), while the cytotoxicity of 6-AZC is essentially lower. The immunocytochemical analysis of virus proteins in the infected cells has shown that 6-AZC inhibits the production of late structural CMV protein gB, but does not influence the expression of early nonstructural protein pp72 and early protein p65.
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